Transcription profiling of mouse colonic epithelial cells treated with sodium butyrate to investigate how sodium butyrate induces arrest of cell cycle and differentiation

ABSTRACT: We performed global scale microarray analysis to identify detailed mechanisms by which sodium butyrate (SB) induce cell growth arrest and differentiation of colonic epithelial cells by using an Affymetrix GeneChip system. Colonic epithelial MCE301 cells used in this study were derived from transgenic mice harboring a tsSV40 large T-antigen. Arrested cell growth and a differentiated phenotype accompanying elevations of alkaline phosphatase activity and histone acetylation were observed in the cells treated with 2 mM SB. Of the 22,690 probe sets analyzed, approximately 2,000 genes were down- and up-regulated by a factor of 2.0 or greater in the cells treated with SB. MCE301 cells were treated with SB for 0, 6, 12 and 24 h. Total RNA samples were prepared from the cells. Gene expression was a

ORGANISM(S): Mus musculus

SUBMITTER: Yoshiaki Tabuchi 

PROVIDER: E-GEOD-4410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress