The histone chaperone Spt6 coordinates histone H3K27 demethylation to regulate gene expression and myogenesis

ABSTRACT: Histone chaperones affect chromatin structure and gene expression through interaction with histones and RNA polymerase II (PolII). Here, we report that the histone chaperone Spt6 counteracts H3K27me3, an epigenetic mark deposited by the Polycomb Repressive Complex 2 (PRC2) and associated with transcriptional repression. We found that Spt6 is required for proper engagement and function of the H3K27 demethylase KDM6A (UTX) on muscle genes and regulates muscle gene expression and cell differentiation. ChIP-Seq experiments revealed an extensive genome-wide overlap of Spt6, PolII and KDM6A at transcribed regions that are devoid of H3K27me3. Mammalian cells and zebrafish embryos with reduced Spt6 display increased H3K27me3 and diminished expression of the master regulator MyoD, resulting in myog

ORGANISM(S): Mus musculus

SUBMITTER: Hossein Zare 

PROVIDER: E-GEOD-44119 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress