Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts [ChIP-seq_histone]

ABSTRACT: MicroRNAs (miRNAs) are critical to proliferation, differentiation, and development. Here, we characterize gene expression in murine Dicer-null adult mesenchymal stem cell lines, a fibroblast cell type. Loss of Dicer leads to de-repression of let-7 targets at levels that exceed 10-100 fold with increases in transcription. Direct and indirect targets of this miRNA belong to a mid-gestation embryonic program that encompasses known oncofetal genes as well as oncogenes not previously associated with an embryonic state. Surprisingly, this mid-gestation program represents a distinct period that occurs between the pluripotent state of the inner cell mass at embryonic day 3.5 and the induction of let-7, upon differentiation, at embryonic day 10.5. Within this mid-gestation program, we character

ORGANISM(S): Mus musculus

SUBMITTER: A Bhutkar 

PROVIDER: E-GEOD-44159 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress