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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression in Drosophila Insensitive mutant

ABSTRACT: Affymetrix arrays measuring gene expression in 3 Drosophila Insensitive mutant embryos and 3 Drosophila wt mutant embryos 6 samples, 3 replicates of Insensitive mutants and 3 replicates of wt

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jakub Westholm

PROVIDER: E-GEOD-44177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The BEN domain is a novel sequence-specific DNA-binding domain conserved in neural transcriptional repressors.

Dai Qi Q Ren Aiming A Westholm Jakub O JO Serganov Artem A AA Patel Dinshaw J DJ Lai Eric C EC

Genes & development 20130306 6

We recently reported that Drosophila Insensitive (Insv) promotes sensory organ development and has activity as a nuclear corepressor for the Notch transcription factor Suppressor of Hairless [Su(H)]. Insv lacks domains of known biochemical function but contains a single BEN domain (i.e., a "BEN-solo" protein). Our chromatin immunoprecipitation (ChIP) sequencing (ChIP-seq) analysis confirmed binding of Insensitive to Su(H) target genes in the Enhancer of split gene complex [E(spl)-C]; however, de ...[more]

PMID: 23468431

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The BEN domain is a novel sequence-specific DNA binding domain conserved in neural transcriptional repressors

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-04-04 | E-GEOD-44178 | biostudies-arrayexpress

Expression data from early and late born Atoh1 lineages within the E14.5 rhombomere 1 and isthmus

Project description:Following neural tube closure at around E9.5, the rhombic lip within the rhombomere 1/isthmus region ("upper rhombic lip") produces a sequence of neuronal lineages that populate the brainstem and cerebellum. The transcription factor Atoh1 (Math1) is required for this specialized neurogenesis, although the genetic programs that delineate the temporal cell fate changes downstream of Atoh1 are not well characterized. We examined the gene expresion changes that take place within Atoh1 lineages We purified early (E10.5) and late (E13.5) born Atoh1 expressing cells from E14.5 embryos using a transgenic labeling strategy, and analyzed differences in gene expression across the two populations using the microarray data shown below.

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Expression profile of Gli1-expressing keratinocytes from mouse skin

Project description:To assess if Hedgehog (Hh) responding cells in the skin have a unique expression profile, isolated keratinocytes that express the Hh response gene Gli1 were collected by FACS and their gene expression was compared to sorted CD34-expressing cells from the middle bulge region of the hair follicle and to cells from the interfollicular epidermis (IFE) by hybridization of isolated RNA to gene expression microarrays. Dissociated keratinocytes isolated form back skin of Gli1-eGFP/+ mice (2 mice pooled for each replicate) in adult telogen were sorted based on GFP expression and immunostaining for CD34. Only viable, single cells with immunostaining for α6 Integrin (to mark basal keratinocytes) were collected. GFP(+) cells were collected as the Gli1 cohort. GFP(-) CD34(+) cells were collected as the CD34 cohort, and GFP(-) CD34(-) cells were collected as interfollicular epidermis (IFE) cohort. Total RNA was extracted from each cell population and labeled for hybridization to gene expression microarrays. The experiment was preformed in triplicate, however RNA from the Gli1(+) cells in one replicate was of insufficient quality to analyze. For staining of isolated keratinocytes, cells were incubated on ice for 1 hour in SMEM containing 1%BSA and 15μL of anti-CD49f-PE antibody (BDBioscience) plus 5μL anti-CD34-APC antibody (eBioscience) for each 100μL total volume for each 2 million cells. Immediately prior to sorting, DAPI (Invitrogen) was added to the washed cells at 1μg/mL final concentration.

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Mouse model of severe asthma: ovalbumin challenge

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2014-02-02 | E-GEOD-27066 | biostudies-arrayexpress

Analysis of tumor- and stroma-supplied proteolytic networks in different metastatic tumor microenvironments identifies a critical role for cathepsin S in brain metastasis

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2014-07-04 | E-GEOD-47930 | biostudies-arrayexpress

Musashi-2 HITS-CLIP sequencing in K562 cells

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2014-10-07 | E-GEOD-62115 | biostudies-arrayexpress

Genome-wide mapping of meiotic double-strand breaks by sequencing Spo11 oligos.

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2011-03-07 | E-GEOD-26449 | biostudies-arrayexpress

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2014-05-12 | E-GEOD-55540 | biostudies-arrayexpress

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