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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from human foreskin fibroblasts (HFFs) infected with type I strains of Toxoplasma gondii.

ABSTRACT: Type I strains of Toxoplasma gondii exhibit phenotypic variation, but it is uncertain how differently type I strains modulate the host cell. We determined differential host modulation by type I strains through microarray. HFFs were infected with RH-ERP, RH-JSR and GT1 for 24 hours. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Ninghan Yang

PROVIDER: E-GEOD-44189 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic basis for phenotypic differences between different Toxoplasma gondii type I strains.

Yang Ninghan N Farrell Andrew A Niedelman Wendy W Melo Mariane M Lu Diana D Julien Lindsay L Marth Gabor T GT Gubbels Marc-Jan MJ Saeij Jeroen P J JP

BMC genomics 20130710

<h4>Background</h4>Toxoplasma gondii has a largely clonal population in North America and Europe, with types I, II and III clonal lineages accounting for the majority of strains isolated from patients. RH, a particular type I strain, is most frequently used to characterize Toxoplasma biology. However, compared to other type I strains, RH has unique characteristics such as faster growth, increased extracellular survival rate and inability to form orally infectious cysts. Thus, to identify candida ...[more]

PMID: 23837824

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Expression data from Human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii.

Project description:Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates the nuclear translocation of the NF-kappaB p65 transcription factor. We used microarrays to determine how GRA15II modulates host cell transcription. HFFs were infected with type I (RH), type I GRA15II (RH GRA15II), type II (Pru), type II GRA15KO (Pru GRA15KO), type III (cep), or type III GRA15II (cep GRA15II) parasites for 18-24 hours. Some samples were also stimulated with TNF-alpha. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.

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Expression data from cyclohexamide treated Human Foreskin Fibroblasts (HFFs) infected with type I Toxoplasma gondii and stimulated with interferon gamma.

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Human foreskin fibroblasts (HFFs) infected with type I strains of Toxoplasma gondii

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LC-MS of interacting partners Toxoplasma gondii GRA57

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