Gene expression in Bacillus subtilis treated with human PGRP, gentamicin, and CCCP

ABSTRACT: Human Peptidoglycan Recognition Proteins (PGRPs) kill bacteria, likely by over-activating stress responses in bacteria. To gain insight into the mechanism of PGRP killing of Bacillus subtilis and bacterial defense against PGRP killing, gene expression in B. subtilis treated with a control protein (bovine serum albumin, BSA), human recombinant PGRP (PGLYRP4), gentamicin (aminoglycoside antibiotic), and CCCP (membrane potential decoupler) were compared. Each treatment induced unique and somewhat overlapping pattern of gene expression. PGRP highly increased expression of genes for oxidative and disulfide stress, detoxification and efflux of Cu, As, and Zn, several transporters, repair of damaged proteins and DNA, energy generation, histidine and cysteine synthesis, envelope lysis and remodeli

ORGANISM(S): Bacillus subtilis

SUBMITTER: Roman Dziarski 

PROVIDER: E-GEOD-44212 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress