PAX5 overexpression is not enough to reestablish the mature B-cell phenotype in classical Hodgkin lymphoma

ABSTRACT: In lymphomas derived from mature B cells the expression of the transcription factor PAX5 is maintained whereas classical Hodgkin lymphoma displays significantly reduced PAX5 expression despite its derivation from mature B cells. To elucidate the functional role of PAX5 in classical Hodgkin lymphoma, we re-established the PAX5 expression in the Hodgkin cell line L428 with and without epigenetic modulation. To this end, we stably transfected the Hodgkin cell line L428 with an inducible PAX5 expression construct. Although the overexpressed PAX5 was transcriptionally active as demonstrated by synthetic reporter constructs, no induction of the B-cell phenotype was achieved. PAX5 chromatin immunoprecipitation with subsequent next generation sequencing in B-cell lines and the PAX5 overexpressing

ORGANISM(S): Homo sapiens

SUBMITTER: Lora Dimitrova 

PROVIDER: E-GEOD-44244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress