Transcription profiling of rat duodenum during immobilization

ABSTRACT: In the present study, we examined duodenal gene expression in rats subjected to immobilization, to elucidate the mechanism of the stress response in the duodenum. Experiment Overall Design: Total RNA was extracted from the duodenal mucosa with an acid guanidinium thiocyanate-phenol-chloroform mixture. Total RNA was analyzed using the rat genome U34A microarray (Affymetrix). Gene intensity information was converted to a mean intensity for each gene using the manufacturer’s proprietary software (version 4.0; Affymetrix), which includes routines for data filtering and normalization.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Tadatoshi Sato 

PROVIDER: E-GEOD-4426 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress