Transcription profiling of rat fibroblasts transformed with HRAS and treated with the demethylating agent 5-aza-2-deoxycytidine

ABSTRACT: Silencing of gene expression by methylation of CpG islands in regulatory elements is frequently observed in cancer. However, an influence of the most common oncogenic signalling pathways onto DNA methylation has not yet been investigated thoroughly. To address this issue, we identified genes suppressed in HRAS-transformed rat fibroblasts but up-regulated after treatment with the demethylating agent 5-Aza-CdR and with the MEK1,2 inhibitor U0126. Analysis of gene expression by microarray and Northern blot analysis revealed the MEK/ERK target genes clusterin, Mmp2, Ppicap, syndecan 4, Timp2, Thbs1 to be repressed in the HRAS-transformed FE-8 cells in a MEK/ERK- and in a methylation-dependent manner. Hypermethylation of putative regulatory elements in HRAS-transformed cells as compared to immo

ORGANISM(S): Rattus norvegicus

SUBMITTER: Christine Sers 

PROVIDER: E-GEOD-4427 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress