Comparison of gene expression in Dnmt1+/+ and Dnmt1-/- MEFs

ABSTRACT: DNA methylation and the Polycomb Repression System are epigenetic mechanisms that play important roles in maintaining transcriptional repression. Recent evidence suggests that DNA methylation can attenuate the binding of Polycomb protein components to chromatin and thus plays a role in determining their genomic targeting. However, whether this role of DNA methylation is important in the context of transcriptional regulation is unclear. By genome-wide mapping of the Polycomb Repressive Complex 2 (PRC2)-signature histone mark, H3K27me3, in severely DNA hypomethylated mouse somatic cells, we show that hypomethylation leads to widespread H3K27me3 redistribution, in a manner that reflects the local DNA methylation status in wild-type cells. Unexpectedly, we observe striking loss of H3K27me3 and

ORGANISM(S): Mus musculus

SUBMITTER: James Reddington 

PROVIDER: E-GEOD-44277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress