Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes [gene expression]

ABSTRACT: The master transcription factors Oct4, Sox2 and Nanog bind enhancer elements and recruit the Mediator co-activator to activate much of the gene expression program of embryonic stem cells (ESCs). We report here that the ESC master transcription factors and Mediator form M-bM-^@M-^\super-enhancersM-bM-^@M-^] at most genes known to control the pluripotent state, including those encoding the master transcription factors themselves. These super-enhancers consist of extraordinarily large genomic domains occupied by exceptional amounts of Oct4, Sox2, Nanog, Klf4, Esrrb and Mediator. Super-enhancers stimulate considerably higher transcription than typical enhancers in vivo and in reporter vectors. Reduced levels of Oct4 or Mediator cause preferential loss of expression of super-enhancer-associat

ORGANISM(S): Mus musculus

SUBMITTER: Richard Young 

PROVIDER: E-GEOD-44287 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress