Rickettsia rickettsii transcriptome - Elevation of temperature response

ABSTRACT: One of the most important vectors of the Brazilian Spotted Fever, the tick Amblyomma aureolatum in Brazil was used in this study. We laboratorial controlled the infection of adult females of A. aureolatum with the virulent brazilian strain Taiacu of Rickettsia rickettsii. The group of ticks was divided into 2 testing groups, group 1 (G1) composed of adult females incubated at 25°C for 3 days and group 2 (G2) composed of adult females incubated at 35°C for 3 days. Right after incubation of both groups, ticks were individually dissected and all internal organs were transferred to RNAlater® Solution (Life Technologies) until gDNA and total RNA simultaneously isolation. A total of 14 ticks of each group were analyzed in two biological replicates (7 ticks each). Dye-swap was also applied to con

ORGANISM(S): Rickettsia rickettsii

SUBMITTER: Maria Galletti 

PROVIDER: E-GEOD-44316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress