Epididymosomes convey different repertoires of miRNAs throughout the epididymis

ABSTRACT: Epididymosomes are small membrane vesicles (50-500nm) secreted by epididymal epithelial cells and involved in post-testicular sperm maturation. While their role in protein transfer to the sperm membrane is well acknowledged, we unveil here their capacity to vehicle microRNAs (miRNAs), which are potent regulators of post-transcriptional gene expression. Using a global microarray approach, we showed that epididymosomes providing from two discrete bovine epididymal regions (caput and cauda) possess distinct miRNA signatures. In addition, we established that miRNA repertoires contained in epididymosomes differ from those of their parent epithelial cells, suggesting that miRNA populations released from the cells may be selectively sorted. Binding of DilC12-labeled epididymosomes to primary cult

ORGANISM(S): Bos taurus

SUBMITTER: Ezequiel Calvo 

PROVIDER: E-GEOD-44365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress