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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Haemophilus ducreyi 35000HPfis relative to 35000HP

ABSTRACT: The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi fis deletion mutant to that of the wild type parental strain. This study includes three biological replicates (paired samples of mutant and wild type strains), and all three were subjected to dye swap.

ORGANISM(S): Haemophilus ducreyi

SUBMITTER: Eric Hansen

PROVIDER: E-GEOD-44413 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Haemophilus ducreyi Fis protein is involved in controlling expression of the lspB-lspA2 operon and other virulence factors.

Labandeira-Rey Maria M Dodd Dana A DA Brautigam Chad A CA Fortney Kate R KR Spinola Stanley M SM Hansen Eric J EJ

Infection and immunity 20130826 11

Expression of the lspB-lspA2 operon encoding a virulence-related two-partner secretion system in Haemophilus ducreyi 35000HP is directly regulated by the CpxRA regulatory system (M. Labandeira-Rey, J. R. Mock, and E. J. Hansen, Infect. Immun. 77:3402-3411, 2009). In the present study, we show that this secretion system is also regulated by the small nucleoid-associated protein Fis. Inactivation of the H. ducreyi fis gene resulted in a reduction in expression of both the H. ducreyi LspB and LspA2 ...[more]

PMID: 23980107

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Haemophilus ducreyi 35000HPhfq relative to 35000HP

Project description:The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi hfq deletion mutant to that of the wild type parental strain. This study includes three biological replicates (paired samples of mutant and wild type strains), and all three were subjected to dye swap.

2014-03-07 | E-GEOD-44535 | biostudies-arrayexpress

Haemophilus ducreyi 35000HPcpxA gene expression relative to 35000HP

Project description:Comparative analysis of the global gene expression of a Haemophilus ducreyi 35000HP cpxA deletion mutant relative to the wild type strain After the cpxA mutant was generated, both strain were grown in Columbia Broth. After 8 hr of growth RNA wa isolated and processed for DNA microarray analysis. This study includes three biological replicates (paired samples), and all three were subjected to dye swap.

2011-05-03 | E-GEOD-21788 | biostudies-arrayexpress

Global gene expression profile of Haemophilus ducreyi 35000HPcpxRpML125 relative to 35000HPcpxRpLS88

Project description:Haemophilus ducreyi 35000HPcpxRpML125 which over-expresses CpxR in a CpxR deletion background, was compared to it vector alone counterpart (35000HPcpxRpLS88) in hopes to elucidate members of the CpxRA regulon. Haemophilus ducreyi 35000HPcpxRpML125 and 35000HPcpxRpLS88 were grown in Columbia broth. After 8 hr of growth, total RNA was isolated and processed for DNA microarray analysis. This study includes 3 biological replicates (paired samples), two of which were selected for dye swap.

2011-05-03 | E-GEOD-21789 | biostudies-arrayexpress

H. ducreyi 35000HP grown in the presence or absence of FCS

Project description:Haemophilus ducreyi 35000HP was subjected to different in vitro growth conditions that resemble the in vivo host environment (presence of serum), in order to ascertain the overall changes in gene expression. This information might identify genes important for colonization and/or survival inside the host. H. ducreyi 35000HP was grown for 8 hours in Columbia broth in the presence or absence of FCS. After 8 hours total RNA was isolated and processed for DNA microarray analysis. This study includes four biological replicates (paired samples of bacteria grown in the presence/absence of FCS), one of which (Rep1) was selected for dye swap.

2010-05-18 | E-GEOD-13851 | biostudies-arrayexpress

Haemophilus ducreyi 35000HPfis relative to 35000HP

Project description:The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi fis deletion mutant to that of the wild type parental strain.

2013-11-04 | GSE44413 | GEO

Haemophilus ducreyi 35000HPhfq relative to 35000HP

Project description:The goal of this study was to compare the global trascription profile of a Haemophilus ducreyi hfq deletion mutant to that of the wild type parental strain.

2014-03-07 | GSE44535 | GEO

Haemophilus ducreyi transcriptome during human infection

Project description:To better understand the molecular mechanisms underlying Haemophilus ducreyi infection in humans, here we determined the transcriptional profile of H. ducreyi in human lesions using RNA-Seq and compared it to that of in vitro growth. We were able to show that the in vivo transcriptome did not resemble that of in vitro growth. Compared to the inoculum, H. ducreyi harvested from pustules differentially expressed ~120 genes, of which 68 were upregulated. A large proportion of the upregulated genes encoded homologs of proteins involved in nutrient transport, alternative carbon pathways, growth arrest response, heat shock response, and DNA recombination. H. ducreyi upregulated few genes or operons (hgbA, flp-tad, and lspB-lspA2) required for human infection; expression of these genes is known to increase under nutrient stress. Homologs of several genes involved in anaerobic metabolism and ascorbate utilization were upregulated in vivo, suggesting that the organism is adjusting its metabolism to anaerobiosis in vivo. RNA from Haemophilus ducreyi infected pustules were collected from four volunteers and performed RNA-Seq.

2015-11-21 | E-GEOD-75236 | biostudies-arrayexpress

Haemophilus ducreyi 35000HPcpxA gene expression relative to 35000HP

Project description:Comparative analysis of the global gene expression of a Haemophilus ducreyi 35000HP cpxA deletion mutant relative to the wild type strain

2011-05-03 | GSE21788 | GEO

[Haemophilus] ducreyi 35000HP

Project description:H. ducreyi 35000HP grown in the presence or absence of FCS

| PRJNA110533 | ENA

Transcriptomes of 35000HP, ÎcpxA, ÎcpxR mutant strains of Haemophilus ducreyi in mid-log, transition, and stationary phase

Project description:To better understand the role of H. ducreyi CpxRA in controlling virulence determinants, here we defined genes potentially regulated by CpxRA by using RNA-Seq. Activation of CpxR by deletion of cpxA repressed nearly 70% of its targets, including seven established virulence determinants. Inactivation of CpxR by deletion of cpxR differentially regulated few genes and increased the expression of one virulence determinant. We identified a CpxR binding motif that was enriched in downregulated but not upregulated targets. These data reinforce the hypothesis that CpxA phosphatase activity plays a critical role in controlling H. ducreyi virulence in vivo. Characterization of the downregulated genes may offer new insights into pathogenesis. RNA of Haemophilus ducreyi wildtype, cpxA and cpxR mutants were collected at mid-log, transition, and stationary phases of growth, in quadruplicate.

2015-11-21 | E-GEOD-75237 | biostudies-arrayexpress

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