Development and application of a microarray for assessing gene transcription in open ocean microbial communities.

ABSTRACT: A high-density oligonucleotide microarray that targets functional genes in marine microbial community was designed as a result of a multi-institutional effort. The design is based on nucleotide sequence data obtained with metagenomics and metatranscriptomics. The chip targets ~20000 gene sequences represented by 145 gene categories relevant to microbial metabolism in the open ocean and coastal environments. The three domains of life and also viruses are represented on the chip. Using this microarray we were able to compare the functional responses of microbial communities to iron and phosphate enrichments in samples from the North Pacific Subtropical Gyre. The response was attributed to individual lineages of microorganisms including uncharacterized strains. Transcription of 68% of the gen

ORGANISM(S): uncultured marine microorganism

SUBMITTER: Irina Shilova 

PROVIDER: E-GEOD-44448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress