Microarray Analysis of Dupuytren's Disease Tissues

ABSTRACT: PURPOSE: Dupuytren's disease (DD) is characterized by fibroblastic proliferation of the palmar fascia, often leading to flexion contracture in the hand. Although there is a strong genetic component the genome-wide expression of novel genes is not known. The purpose of this study was to use DNA microarray technology to identify upregulated genes in DD. METHODS: Human tissue samples were harvested from 3 patient sources: DD cord tissue (n = 20), normal-appearing adjacent control fascia (n = 15), and palmar fascia from patients having carpal tunnel release (n = 15). DNA microarray analysis was performed on amplified sample RNA. Novel genes were compared with known gene functions. A candidate gene of interest was studied further by using immunohistochemistry on DD tissue samples and controls.

ORGANISM(S): Homo sapiens

SUBMITTER: Kenton Fong 

PROVIDER: E-GEOD-4457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress