Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

ABSTRACT: The degradation of TNFM-NM-1 mRNA is critical for restricting TNFM-NM-1 synthesis and involves a constitutive decay element (CDE) in the 3M-bM-^@M-^Y untranslated region of the mRNA. We demonstrate that the active CDE is a stem-loop RNA motif that is recognized by the RNA-binding protein Roquin. Deep sequencing of Roquin-associated mRNAs identified CDE-containing mRNAs as the primary targets of Roquin on a transcriptome-wide scale. Expression profiles of LPS treated Raw264.7 cells (input), after Roquin IP or control IP were generated by deep sequencing, in three biological replicates, using Illumina HiSeq 2000.

ORGANISM(S): Mus musculus

SUBMITTER: Georg Stoecklin 

PROVIDER: E-GEOD-44775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress