Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Bovine mammary gene expression profiling during the onset of lactation


ABSTRACT: Lactogenesis includes two stages. Stage Ⅰ begins a few weeks before parturition, and stage Ⅱ begins a few weeks before parturition. In order to better understand the molecular events driving lactogenesis in dairy cows, genome-wide gene expression profiling was conducted using digital gene expression profiling (DGE) on bovine mammary tissue at three time points (on approximately day 35 before parturition (-35d), day 7 before parturition (-7d) and day 3 after parturition (+3d)). Approximately 6.2 million, 5.8 million and 6.1 million 21-nt cDNA tags were sequenced in the three cDNA libraries (-35d, -7d and +3d), respectively. After aligning to the reference sequences, the three cDNA libraries included 8,662, 8,363 and 8,359 genes, respectively. With a fold change cutoff criteria of ≥2 or ≤-2 and a false discovery rate (FDR) of ≤0.001, a total of 812 genes were significantly differentially expressed at -7d compared with -35d (stage Ⅰ). There were 234 genes upregulated and 578 genes downregulated, accounting for 28.8% and 71.2% of differentially expressed genes respectively. A total of 1,189 genes were significantly differentially expressed at +3d compared with -7d (stag Ⅱ). Of these genes with significant expression changes, 274 (23.0%) genes were upregulated. 915 (77.0%) genes were downregulated. The results suggest that the mammary gland begins to lactate not only by a gain of function but also by a broad suppression of function to effectively push most of the cell's resources towards lactation. Examination of genome-wide gene expression profiling using digital gene expression profiling (DGE) on bovine mammary tissue at three time points of approximately day 35 before parturition, day 7 before parturition and day 3 after parturition. There were 6 cows in each group. The mammary tissue from cows in the same group were pooled together for RNA isolation. The total RNA was used to sequence.

ORGANISM(S): Bos taurus

SUBMITTER: yuanyuan gao 

PROVIDER: E-GEOD-44796 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2016-03-01 | GSE44796 | GEO
2015-09-01 | GSE65059 | GEO
2021-01-16 | GSE164924 | GEO
2017-12-31 | GSE79692 | GEO
2012-02-27 | E-GEOD-27380 | biostudies-arrayexpress
2012-03-01 | E-GEOD-19055 | biostudies-arrayexpress
2007-12-22 | E-GEOD-9344 | biostudies-arrayexpress
2013-07-11 | GSE48685 | GEO
2012-02-27 | GSE27380 | GEO
2021-01-16 | GSE164923 | GEO