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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

ABSTRACT: Seasonal photoperiodic changes have strong impact on development in Nasonia vitripennis. Here, Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps of female wasps maintained in long vs short day. We have identified differential methylated loci that encode the photoperiodic change. analysis of DNA methylation in female wasps maintained in long vs short day, using RRBS followed by Illumina sequencing

ORGANISM(S): Nasonia vitripennis

SUBMITTER: Eran Tauber

PROVIDER: E-GEOD-44869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

Project description:Seasonal photoperiodic changes have strong impact on development in Nasonia vitripennis. Here, Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps of female wasps maintained in long vs short day. We have identified differential methylated loci that encode the photoperiodic change.

2014-09-01 | GSE44869 | GEO

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Tet and TDG Mediate DNA Demethylation Essential for MET in Somatic Cell Reprogramming

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2016-08-02 | E-GEOD-84103 | biostudies-arrayexpress

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Project description:We quantified the targets and kinetics of DNA methylation acquisition in mouse embryos, and determined the contribution of the de novo methyltransferases DNMT3A and DNMT3B to this process. We provide single-base maps of cytosine methylation by RRBS from the blastocysts to post-implantation stages and in embryos lacking DNMT3A or DNMT3B activity, and performed RNA-Seq in embryos lacking DNMT3B activity. We sequenced RRBS libraries prepared from genomic DNA isolated from embryos at consecutive stages of development between E3.5 and E11.5,and adult differentiated cells (sperm, liver). We performed RRBS on blastocysts at E3.5/E4.5, dissected epiblasts at E5.5/E6.5/E7/5, whole embryos at E8.5/E10.5 and limbs at E11.5. RRBS experiments in Dnmt3a-/- and Dnmt3b-/- embryos were performed in biological duplicates on individual embryos. We sequenced RNA-Seq libraries prepared from total RNAs of three WT and Dnmt3b-/- littermate embryos collected at E8.5.

2014-11-14 | E-GEOD-60334 | biostudies-arrayexpress

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Project description:DNA methylation profiling using RRBS in wild type and Dppa3 knockout ESCs.

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Methylation disorder in CLL

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2015-01-01 | E-GEOD-58889 | biostudies-arrayexpress

FBXL10 and the Protection of Polycomb-bound Genes from Hypermethylation (RRBS)

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DNA methylation changes induced by overexpression of IDH1mut or treatment with 2HG in the sorted mouse bone marrow cells

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