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Interleukin-27 treated human macrophages induce the expression of novel miRNAs which may mediate anti-viral properties (RNA-seq)

ABSTRACT: In this study, we hypothesized that IL-27 could induce the expression of novel miRNAs in macrophages which may have functional relevance in terms of anti-viral activity. In this study, primary monocytes were differentiated into macrophages using M-CSF (M-Mac) or with a combination of M-CSF and IL-27 (I-Mac) for seven days. Following this, total RNA was extracted from these cells and deep sequencing was performed, in parallel with gene expression microarrays. Using the novel miRNA discovery software, miRDeep, seven novel miRNAs were discovered in the macrophages, four of which were expressed higher in I-Mac (miRNAs 2.1, 8.1, 9.1 and 14.2) whilst three were detected in both M-Mac and I-Mac (miRNAs 9.3, 13.6 and 15.8). The expression of six of the seven novel miRNAs was highly correlated with qRT-PCR using specific primer/probes designed for the novel miRNAs. Gene expression microarray further demonstrated that a number of genes were potentially targeted by these differentially expressed novel miRNAs. screening novel and known miRNAs which may have antiviral properties in 2 different treatments in 2 donors.

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaojun Hu

PROVIDER: E-GEOD-44953 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Interleukin-27 treated human macrophages induce the expression of novel miRNAs which may mediate anti-viral properties (affy)

Project description:In this study, we hypothesized that IL-27 could induce the expression of novel miRNAs in macrophages which may have functional relevance in terms of anti-viral activity. In this study, primary monocytes were differentiated into macrophages using M-CSF (M-Mac) or with a combination of M-CSF and IL-27 (I-Mac) for seven days. Following this, total RNA was extracted from these cells and deep sequencing was performed, in parallel with gene expression microarrays. Using the novel miRNA discovery software, miRDeep, seven novel miRNAs were discovered in the macrophages, four of which were expressed higher in I-Mac (miRNAs 2.1, 8.1, 9.1 and 14.2) whilst three were detected in both M-Mac and I-Mac (miRNAs 9.3, 13.6 and 15.8). The expression of six of the seven novel miRNAs was highly correlated with qRT-PCR using specific primer/probes designed for the novel miRNAs. Gene expression microarray further demonstrated that a number of genes were potentially targeted by these differentially expressed novel miRNAs. Gene expression microarrays from 3 samples of microphage treated with M-CSF (M-MAC) were compared with 3 samples of micropahge treated with M-CSF + IL-27 (I-MAC)

2013-03-29 | E-GEOD-44955 | biostudies-arrayexpress

Interleukin-27 treated human macrophages induce the expression of novel miRNAs which may mediate anti-viral properties (RNA-seq)

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Interleukin-27 treated human macrophages induce the expression of novel miRNAs which may mediate anti-viral properties (affy)

2013-03-29 | GSE44955 | GEO

Expression analysis of dendritic cells, macrophages and monocytes

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Interleukin-27 treated human macrophages induce the expression of novel miRNAs which may mediate anti-viral properties

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-29 | E-GEOD-44957 | biostudies-arrayexpress

Sublytic membrane attack complex drives glycolysis and mitochondrial dysfunction with inflammatory consequences in human monocyte-derived macrophages

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Transcriptomics comparisons of Mac-T cells versus mammary tissue during late pregnancy and peak lactation

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HUVEC endosome LCMS - ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes

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2019-04-09 | PXD013381 | Pride

Mechano-redox control of Mac-1 de-adhesion from ICAM-1 by protein disulfide isomerase promotes directional movement under flow

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Profiling microRNA expression in bovine alveolar macrophages using RNA-seq

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