Age-Related Changes Following in vitro Stimulation with Rhodococcus equi of Peripheral Blood Leukocytes from Neonatal Foals

ABSTRACT: The objectives of this study were 2-fold: 1) to compare the expression profiles at specific ages of blood leukocytes from foals stimulated with virulent R. equi with those of unstimulated leukocytes; and, 2) to characterize the age-related changes in the gene expression profile associated with blood leukocytes in response to stimulation with virulent R. equi. Peripheral blood leukocytes were obtained from 6 foals within 24 hours (h) of birth (day 1) and 2, 4, and 8 weeks after birth. The samples were split, such that half were stimulated with live virulent R. equi, and the other half served as unstimulated control. For one hyb design: we hybridized 6 samples stimulated and 6 unstimulated at each time point (30 mL of blood was treated with live virulent R. equi (Strain ATCC 33701) and the r

ORGANISM(S): Equus caballus

SUBMITTER: Priyanka Kachroo 

PROVIDER: E-GEOD-45004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress