Transcription profiling of mouse oocytes reveals abundant transcripts from retrotransposons are unstable

ABSTRACT: One physiological function proposed for RNA interference (RNAi) is to constrain expression of repetitive elements and thereby reduce the incidence of retrotransposition. Consistent with this model is that inhibiting the RNAi pathway results in an increase in expression of repetitive elements in preimplantation mouse embryos. Mouse oocytes are essentially transcriptionally quiescent providing a unique opportunity to assess the stability of repetitive element-derived transcripts in these cells. We compared the transcriptome of freshly isolated fully-grown germinal vesicle (GV)-intact oocytes to that of oocytes in which meiotic maturation in vitro was inhibited for 48 h by milrinone. Consistent with the aforementioned function for RNAi is that the abundance of only a relatively small numb

ORGANISM(S): Mus musculus

SUBMITTER: Mareike Puschendorf 

PROVIDER: E-GEOD-4508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress