Mutational activation of a mechanosensitive megachannel drives metastatic cell survival.

ABSTRACT: Next-generation sequencing has revolutionized cancer biology by accelerating the unbiased discovery of novel mutations across human cancers. Transforming such discoveries into a conceptual framework of cancer progression requires narrowing the vast number of mutations down to the driver elements, and further reducing these to mutations that govern cancer progression as opposed to tumor initiation. By integrating next-generation RNA-sequencing (RNA-seq) with in vivo selection, we devise an approach that identifies a series of novel recurrent non-synonymous amino acid mutations that are enriched in metastatic breast cancer cells and predicted to significantly alter protein function. These mutations, found in PANX1, RBFA, REST, KRIT1 and ZSWIM6, are detected at higher frequencies in the trans

ORGANISM(S): Homo sapiens

SUBMITTER: David Soong 

PROVIDER: E-GEOD-45162 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress