Transcription profiling of mouse expressing non-cleavable transmembrane mutant form of murine TNFalpha, or treated with soluble TNFalpha (20ng/ml) for 4 hours to investigate divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation

ABSTRACT: Activation of the vascular endothelium with cytokines such as TNF is widely used to study the role of the vasculature in proinflammatory disease. To gain insight into mechanisms of prolonged vascular endothelial activation we compared changes in gene expression induced by continuous activation in stable tmTNFalpha expressing cells with changes due to acute TNFalpha challenge in vitro. Affymetrix Genechip® analysis was performed on RNA from control, acute and continuous TNFalpha-activated endothelial cells. Only 36% of the significant changes in gene expression were convergent between the acute and continuously activated endothelial cells in comparison to the control. From the divergently regulated genes, for example the cytokine ENA-78 was specifically induced in chronically activated ce

ORGANISM(S): Mus musculus

SUBMITTER: Matthew Grow 

PROVIDER: E-GEOD-4518 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress