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Unknown,Transcriptomics,Genomics,Proteomics

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Growth factor stimulation of cardiomyocytes induces changes in the transcriptional contents of secreted exosomes

ABSTRACT: To study if the transcriptional content in exosomes derived from unntreated and growth factor treated cultured cardiomyocytes (HL-1) differ and if so, can this difference be explained.This is results for the cells.The exosome reults can be find at GSE40503. 4 control untreated samples, 4 TFG-beta2 cardiomyocyte treated samples and 4 PDGF BB cardiomyocyte were studied., this reults are from the corresponding cells (that released the exosomes), used for data filtering of the exsomedata. The exosome reults can be find at GSE40503.

ORGANISM(S): Mus musculus

SUBMITTER: Nina Gennebäck

PROVIDER: E-GEOD-45207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Study of transcriptional content in exosomes derived from untreated and growth factor-treated cultured cardiomyocytes (HL-1)

Project description:Introduction: Exosomes are nano-sized extracellular vesicles, released from various cells, which can stimulate or repress responses in target cells. We have recently shown that cultured cardiomyocytes release exosomes and that they, in turn, are involved in facilitating events in target cells by alteration of gene expression. We investigated whether external stimuli of the cardiomyocyte might influence the released exosome characteristics. Material and Methods: Exosomes were isolated from media collected from cultured cardiomyocyte (HL-1) cells with or without growth factor treatment (TGF-beta2 and PDGF-BB), with a series of differential centrifugations. The exosomes were characterized with dynamic light scattering (DLS) and Western blot and analysed with Illumina whole genome microarray gene expression. Results: An average size of 50-80 nm in diameter with no difference between treatment groups was found. Analysis of the mRNA content revealed 623 transcripts in the control group, 691 in the TGF-beta2-treated group and 362 in the PDGF-BB-treated group. 235 transcripts were common for all three groups. Conclusion: We conclude that there is a difference in mRNA content between exosomes derived from cultured cardiomyocytes stimulated with growth factors. We also conclude that all exosomes contain a basic package consisting of ribosomal transcripts and mRNAs coding for proteins with functions within the energy supply system. To study if the transcriptional content in exosomes derived from untreated and growth factor-treated cultured cardiomyocytes (HL-1) differ, and if so, can this difference be explained, 4 control (untreated) exosome samples, 4 TFG-beta2-treated cardiomyocyte-derived exosome samples and 4 PDGF-BB-treated cardiomyocyte-derived exosomes were studied.

2013-06-20 | E-GEOD-40503 | biostudies-arrayexpress

Gene Expression in the Human Mammary Epithelium in response to consumption of high fat (H-FAT) and high carbohdyrate (H-CHO) diets.

Project description:Mammary gland (MG) de novo lipogenesis contributes significantly to milk fat in animals but little is known in humans. We hypothesized that de novo lipogenesis, as reflected by incorporation of 13C carbons from [U-13C]glucose into fatty acids (FAs) and glycerol in triglycerides (TG), will be greater: a. in milk than plasma TG, b. during a high carbohydrate (H-CHO) diet than high fat (H-FAT) diet and c. during feeding than fasting. Healthy lactating women were studied on two isocaloric, isonitrogenous diets. On one occasion subjects received diets containing H-FAT or H-CHO diet for 1 week. Incorporation of 13C from infused [U-13C]glucose into FAs and glycerol was measured using GC/MS methodology and gene expression using RNA isolated from breast milk fat globule (MFG). Incorporation of 13C2 into milk FAs, increased with increased chain length of the FAs from C2:0 to C12:0 but progressively declined in C14:0 and C16:0 and was not detected in FAs >C16. During feeding, regardless of diets, enrichment of 13C2 in milk FA and 13C3 in milk glycerol were ~3 and ~7 fold higher compared to plasma FA and glycerol, respectively. Following an overnight fast during H-CHO and H-FAT diets study periods, 25% and 6%, respectively, of medium chain FAs (MCFAs, C6-C12) were derived from glucose but increased to 75% and 25% with feeding. The expression of genes involved in FA or glycerol synthesis pathways was unchanged regardless of diet or fast-fed conditions. Conclusions: The human MG is capable of de novo lipogenesis, of primarily MCFAs and glycerol, which is influenced by the macro-nutrient composition of the maternal diet. On day 7 of consumption of each of the two diets milk samples collected from 7 healthy, lean, exclusively breastfeeding women following an overnight fast and feeding conditions [7 x 2 x 2 = 28 samples] were processed for isotope enrichments and RNA isolation from the milk fat globules. The total RNA was utilized for microarray analyses.

2014-05-19 | E-GEOD-51874 | biostudies-arrayexpress

Gadd45a and Ing1 interaction in epigenetic gene regulation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-02-04 | E-GEOD-43319 | biostudies-arrayexpress

Gene expression in liver tissues derived from wild-type and Siglec-E-deficient mice at 55 weeks of age

Project description:We show that mice lacking Siglec-E, the main member of the CD33rSiglec family, exhibit reduced survival. Removal of Siglec-E causes the development of exaggerated signs of aging at the molecular, structural, and cognitive level. We found that accelerated aging was related both to an unbalanced ROS metabolism, and to a secondary impairment in detoxification of reactive molecules, ultimately leading to increased damage to cellular DNA, proteins and lipids. Taken together, our data suggest that CD33rSiglecs co-evolved in mammals to achieve a better management of oxidative stress during inflammation, which in turn reduces molecular damage and extends lifespan. Total RNA obtained from liver tissues from two wild-type and three Siglec-E-deficient mice

2016-01-15 | E-GEOD-64760 | biostudies-arrayexpress

Long-term maintenance time-course of cardiomyocytes differentiated from human pluripotent stem cells and comparison to human heart

Project description:Analysis of culture-induced changes in cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs) over a time-period of 8 weeks, and comparison of these samples to human atrial and ventricular tissue Total RNA isolated from differentiated in-vitro samples at the indicated time-points and of human heart in-vivo tissue

2014-12-16 | E-GEOD-64189 | biostudies-arrayexpress

Investigation of signaling requirements underlying cardiac induction of human pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-11-12 | E-GEOD-67180 | biostudies-arrayexpress

Histone posttranslational modifications and cell fate determination: Lens induction requires the lysine acetyltransferases CBP and p300

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-09-23 | E-GEOD-49219 | biostudies-arrayexpress

Gene expression changes in HEK293T cells upon overexpression of ING1b and GADD45a

Project description:ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We found that ING1b and GADD45a physically and functionally interact in the epigenetic regulation of specific target genes. In order to characterise the functional ING1b-GADD45a interaction, we performed a gain-of-function experiment in HEK293T cells by individual and combinatorial plasmid transfections and then analysed the transcriptional response via expression microarray profiling. HEK293T cells were transiently transfected with expression plasmids encoding human GADD45a and/or human ING1b (full-length or without its PHD-domain) and harvested 48h post-transfection for Illumina microarray profiling. Two independently transfected replicate samples of each condition were analysed. Empty vector (control) transfections served as reference samples.

2013-02-04 | E-GEOD-43317 | biostudies-arrayexpress

Gene expression changes in mouse embryonic fibroblasts (MEF cells) deficient for Ing1 and Gadd45a

Project description:ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We found that ING1b and GADD45a physically and functionally interact in the epigenetic regulation of specific target genes. In order to study this interaction further, we analysed the transcriptional changes in MEF cells from single and double Ing1/Gadd45 knockout mice via microarray profiling. Mouse embryonic fibroblasts (MEF cells) were isolated from embryonic day E15.5 male embryos, either wild-type (WT) or knockout for Ing1 (Ing1-/-), Gadd45a (Gadd45a-/-) or Ing1/Gadd45a (double knockout, DKO), and cultured for 3 passages. Samples were then collected in duplicates per MEF line for expression array profiling.

2013-02-04 | E-GEOD-43318 | biostudies-arrayexpress

Rapid changes of gene expression in rat adipose tissue when food was removed

Project description:When food was removed for 6 hours, 43 genes, including Angptl4, changed their expression more than two-fold. Food was removed from young rats at 07:30. Six hours later, samples of epididymal adipose tissue was taken for analysis.

2012-12-01 | E-GEOD-41800 | biostudies-arrayexpress

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