Defining a molecular roadmap of cellular reprogramming into iPS Cells [DNA methylation]

ABSTRACT: Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is inefficient, complicating mechanistic studies. Here, we examined defined intermediate cell populations poised to becoming iPSCs by genome-wide analyses. We show that induced pluripotency elicits two transcriptional waves, which are driven by c-Myc/Klf4 (first wave) and Oct4/Sox2/Klf4 (second wave). Cells that become refractory to reprogramming activate the first but fail to initiate the second transcriptional wave and can be rescued by elevated expression of all four factors. The establishment of bivalent domains occurs gradually after the first wave, whereas changes in DNA methylation take place after the second wave when cells acquire stable pluripotency. This integrative analysis allowed us to i

ORGANISM(S): Mus musculus

SUBMITTER: Ben Wittner 

PROVIDER: E-GEOD-45248 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress