CD34 negative populations are associated with an increased relapse risk in childhood AML and harbor chemo-resistant leukemia stem cells

ABSTRACT: Differences in chemo-sensitivity of subpopulations of AML stem cells could have important clinical implications. Using in vitro cytotoxicity, xenograft models and colony forming assays, we compared chemotherapy sensitivity between Lineage (Lin-)CD34-CD38-, Lin-CD34-CD38+, Lin-CD34+CD38- and Lin-CD34+CD38+ populations from 26 primary AMLs (19 paediatric and 7 adult). We identified a common recurring pattern of chemo-response associated with a poor clinical outcome: In each of 16/26 (62%) AMLs, Lin-CD34-CD38- cells were the most chemoresistant of the four subpopulations to daunorubicin in vitro. Cytarabine-resistant colonies formed only from Lin-CD34-CD38- populations following tertiary passages through both NOG mice and methylcellulose in these AMLs The presence of chemo-resistant Lin-CD34-

ORGANISM(S): Homo sapiens

SUBMITTER: Wenbin Wei 

PROVIDER: E-GEOD-45249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress