Digital expression profiling identifies CDC5L, RECQL4, and CDK4 as potential oncogenes in a cohort of pediatric osteosarcoma tumours

ABSTRACT: Osteosarcoma is the most common malignancy of bone, and occurs most frequently in children and adolescents. Currently, the most reliable technique for prognostication is measuring histopathologic tumor necrosis following preoperative chemotherapy, and favourable prognosis is signified by 90% or greater estimated necrosis of the tumour. Neither genetic testing nor molecular biomarkers have been described for this tumour. We used the novel nanoString mRNA expression analysis system to analyzed total RNA from 35 flash-frozen sporadic paediatric osteosarcoma biopsy and resection specimens to quantify mRNA expression for 17 oncogenes and tumour suppressor genes. Three oncogenes, cell cycle regulator gene CDC5L, the RecQ DNA helicase gene RECQL4, and the cyclin-dependent kinase gene CDK4, were m

ORGANISM(S): Homo sapiens

SUBMITTER: Jeff Martin 

PROVIDER: E-GEOD-45275 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress