Transposon defense by endo-siRNAs, piRNAs and somatic pilRNAs: Roles of Loqs-PD and R2D2

ABSTRACT: Transposable elements are a serious threat for genome integrity and their control via small RNA mediated silencing pathways is an ancient strategy. The fruit fly Drosophila melanogaster has two silencing mechanisms that repress TEs expression: endogenous siRNAs (esiRNAs or endo-siRNAs) and Piwi-interacting small RNAs (piRNAs). The biogenesis of endo-siRNAs involves Loqs-PD, which acts predominantly during processing of dsRNA by Dcr-2, and R2D2 that primarily helps to direct siRNAs for loading into Ago2. We provide deep sequencing evidence consistent with the idea that R2D2 and Loqs-PD can function in part redundantly. Certain transposons display a preference for either dsRBD-protein for production or loading; this appeared to correlate neither with overall abundance, classification of the

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Klaus Förstemann 

PROVIDER: E-GEOD-45290 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress