Yeast zeta-crystallin: An AU-Rich Element Binding Protein Involved in the Nutritional Stress Response

ABSTRACT: The yeast M-NM-6-crystallin Zta1p interacts specifically with RNA sequences rich in adenine and uracil (AU-rich element). We have looked for possible targets of Zta1p through a comparative transcriptional analysis of the yeast defective in ZTA1 (M-NM-^Tzta1) versus the wild-type by microarray. This revealed that a group of genes implicated on amino acid biosynthesis are affected by the lack of ZTA1. The effect on one of the targets ARG4 was studied in more detail, revealing a clear positive correlation between ZTA1 and ARG4 expression at the mRNA level. We demonstrate that Zta1p is able to bind specifically to the 3M-bM-^@M-^YUTR AU-rich elements of ARG4 mRNA in vitro, suggesting that the mechanism by which ZTA1 modulates the expression of ARG4 is probably mediated by this mRNA binding abi

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Lauro Sumoy 

PROVIDER: E-GEOD-45297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress