RNAseq analysis of the serotype M1 group A Streptococcus strain MGAS2221

ABSTRACT: We recovered RNA from exponential and stationary phase cell cultures of MGAS2221 and utilized two distinct methods to remove rRNA from our samples, the RiboZero kit from Epicenter, and the terminator- 5--Phosphate-Dependent Exonuclease (TEX). This gave us 4 distinct RNAs with which we performed RNAseq analysis. Both rRNA removal methods worked well, with only a small percentage of the final total read counts being derived from rRNA sequences. A single group A Streptococcus strain was investigated.

ORGANISM(S): Streptococcus pyogenes

SUBMITTER: Paul Sumby 

PROVIDER: E-GEOD-45325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress