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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Differential gene expression during Exobasidium vexans and Pestalotiopsis theae infection in tea by DNA Microarray

ABSTRACT: Microarray analysis is being performed with cultivated selections using custom designed arrays. Custom designed arrays include the design of microarray probes using clearly described bioinformatics methods. We have used the sequence data related to fungal resistance from Camellia Sp. and Arabidopsis thaliana available in the biological databases, to design these arrays. Results of this work will help us to understand the genes expressed during the blister blight and grey blight infection. Camellia sinensis 4x44k Microarray designed by Genotypic Technology Private Limited. (AMADID:043117)

ORGANISM(S): Camellia sinensis

SUBMITTER: Genotypic technology

PROVIDER: E-GEOD-45360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global gene expression profile of Japanese encephalitis virus(JEV) infected cytolytic B16F10 and Non-cytolytic JEV resistant B16F10r

Project description:B16F10, a murine melanoma cel line is cytolytic to JEV infection. We have identified a B16F10 cell line variant which is non-cytolytic to JEV infection. This variant cell line has two types of cells, one which is persistently infected JEV and another which is resistant to JEV infection. The JEV-resistant cells (B16F10r) were seperated from the presistently infected cells by single cell cloning. To understand the mechanism of JEV resistance microarray analysis was caried out to Identify and characterize genes differentially expressed during in uninfected/JEV-infected B16F10 and B16F10r cells. Agilent one-color experiment,Organism: Mus musculus ,Agilent Custom Mouse Whole Genome Mouse 8x60k Gene expression designed by Genotypic Technology Private Limited, Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

2013-08-01 | E-GEOD-42942 | biostudies-arrayexpress

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Project description:Reptiles are phylogenically important group of animals as mammals are evolved from them. Clearly distinct phases of reproductive cycle in wall lizard enable us to study mechanisms regulating spermatogenesis. We have performed microarray hybridizations on mouse whole genome 60K gene chip using RNA from regressed, recrudescent and active phase of reproductive cycle. Microarray data was analyzed considering regressed phase as control. To the best of our knowledge, this is the first report of transcriptome analysis of whole testis of wall lizard from various phases of reproductive cycle. Microarray results reveal that maximum number of differential gene expression is in between regressed and recrudescent phase than in between recrudescent and active phase. The genes prominently expressed in recrudescent and active phase testis are cytoskeleton organization, cell growth and division, osmo-regulation, differentiation and apoptosis. The genes showing higher expression in regressed phase belonged to functional categories such as negative regulation of growth, repression of transcription, maintenance of stem cell niche and muscle related proteins. Summarily, this is first exploratory study profiling transcriptome of three drastically different conditions of wall lizard testis. This study will pave the way for deeper insight into gene regulatory mechanisms in spermatogenesis and evolution of spermatogene processes. Agilent one-color experiment,Organism: Mouse ,Agilent-Custom Whole Genome Mouse 8x60k designed by Genotypic Technology Pvt. Ltd. (AMADID: 026986) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

2013-03-01 | E-GEOD-36505 | biostudies-arrayexpress

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2012-10-01 | E-GEOD-32447 | biostudies-arrayexpress

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2013-08-01 | E-GEOD-39810 | biostudies-arrayexpress

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Project description:A microarray study including two primary isolated human myoblast cell cultures ( samples A and B) was designed in order to detect the differences in global gene expression during four different culture conditions, two actively proliferating and two non-proliferating. Myoblasts were kept long term proliferating (BG0, before G0) followed by G0 arrest (G0), reactivation (AG0, after G0) and finally they were differentiated (Dif). The samples BG0 and AG0 were harvested when cells reached 70-80% confluence. The raw data from gene arrays were normalized, sample A and B were paired and the mean expression value used for further analyses. The four samples were compared to each other in order to detect differences in gene expression between the samples. Primary isolated human myoblast cells were cultured through four different culture, BG0, G0, AG0 and Dif. Two independent studies were made with primary isolated myoblasts from two different individuals for each of the four culture condition. Thus the number of samples for microarray analyses was 8.

2012-09-01 | E-GEOD-38769 | biostudies-arrayexpress

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