Whole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to MycobacteriumM-BM- bovis infection in vitro

ABSTRACT: Background: MycobacteriumM-BM- bovis, the causative agent of bovine tuberculosis, is an intracellular pathogen that can persist inside host macrophages during infection via a diverse range of mechanisms that subvert the host immune response. In the current study, we have analysed and compared the transcriptomes of M.M-BM- bovis-infected monocyte-derived macrophages (MDM) purified from six Holstein-Friesian females with the transcriptomes of non-infected control MDM from the same animals over a 24 h period using strand-specific RNA sequencing (RNA-seq). In addition, we compare gene expression profiles generated using RNA-seq with those previously generated by us using the high-density AffymetrixM-BM-. GeneChipM-BM-. Bovine Genome Array platform from the same MDM-extracted RNA. Results: A me

ORGANISM(S): Bos taurus

SUBMITTER: David MacHugh 

PROVIDER: E-GEOD-45439 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress