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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiles of unfertilized eggs of Ciona intestinalis

ABSTRACT: The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays. To obtain gene expression profiles of unfertilized eggs, we took 14 biological replicates. For the first through fourth replicates, we also took a technical duplicate. Gene expression profiles of the 8-, 16-, 32-cell embryos were obtained with embryos from the third to fifth animals.

ORGANISM(S): Ciona intestinalis

SUBMITTER: Yutaka Satou

PROVIDER: E-GEOD-45500 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome dynamics in early embryos of the ascidian, Ciona intestinalis.

Matsuoka Terumi T Ikeda Tatsuro T Fujimaki Kotaro K Satou Yutaka Y

Developmental biology 20131009 2

Maternally provided mRNAs and proteins direct early development and activate the zygotic genome. Using microarrays, we examined the dynamics of transcriptomes during the early development of a basal chordate, Ciona intestinalis. Microarray analysis of unfertilized eggs, as well as 8-, and 16- and 32-cell embryos revealed that nearly half of the genes encoded in the genome were expressed maternally, and that approximately only one-fourth of these genes were expressed at similar levels among eggs ...[more]

PMID: 24120375

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