Genome-wide analysis of condensin binding sites in vertebrate genome

ABSTRACT: The condensin complex is well known to be essential for correct packaging and segregation of chromosomes during mitosis and meiosis in all eukaryotes. To date, the genome wide location and the nature of condensin binding sites has remained elusive in vertebrate systems. A detailed knowledge of condensin binding sites is essential to understand the as-yet enigmatic function of this complex and to define its greater role in chromosome architecture. To address this, we have used next generation sequencing to map condensin I in chicken DT40 cells. Unexpectedly, we found condensin I binds predominately to promoter sequences in mitotic cells. We also found a striking enrichment at both centromeres and telomeres, highlighting the importance of the complex in chromosome segregation. Taken together

ORGANISM(S): Gallus gallus

SUBMITTER: Ji Hun Kim 

PROVIDER: E-GEOD-45552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress