Regulation by growth temperature of Pseudomonas aeruginosa quorum-sensing depedent virulence factors production involves two RNA-thermometers.

ABSTRACT: Several bacterial human pathogens regulate the production of virulence factors by temperature, expressing them only at 37 M-BM-0C. Accordingly we show that the production of all P. aeruginosa virulence factors that are dependent on the QS transcriptional regulator RhlR, but only a fraction that are activated by LasR, are induced at 37 M-BM-0C compared to 30 M-BM-0C or 25 M-BM-0C. The RhlR-dependent induction at 37 M-BM-0C is a posttranscriptional effect due to an RNA thermometer of the ROSE family that thermoregulates the expression of rhlAB operon involved in rhamnolipids production, a virulence associated trait. This RNA structure also affects the expression of the downstream rhlR gene. A second thermometer is present upstream lasI and causes a reduced expression of this gene at lower te

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Victoria Grosso 

PROVIDER: E-GEOD-45695 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress