A new approach for the study of lung smooth muscle phenotypes and its application in asthma

ABSTRACT: We developed a new method for the isolation of bronchial smooth muscle cells. This method is built upon a double fluorescent mouse SMA-GFP;NG2-DsRed and cell sorting. Using this method, we isolated bronchial smooth muscle cells from control and asthmatic C57BL/6J mice for gene profiling. Double fluorescent mice were induced to develop asthma using OVA. Bronchial smooth muscle cells were purified from asthmatic and control mice for mRNA array.

ORGANISM(S): Mus musculus

SUBMITTER: Adam Gower 

PROVIDER: E-GEOD-45723 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress