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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression data from MCF-7 cells treated with Lacciac Acid A

ABSTRACT: Lacciac Acid A was indentified as an inhibitor of DMNT1. MCF-7 cells were treated with Lacciac Acid A (200 uM) for 5 days. Changes in gene expression were identified by using Affymetrix Human gene ST1.0 arrays. We used microarrays to determine global changes in gene expression upon treatment with Lacciac Acid A an inhibitor of DMNT1. MCF-7 cells near mid passage were treated with Lacciac Acid A for 5 days. RNA was extracted and hybridized on Affymetrix microarrays. Treated and untreated cells were from the same cell passage. Two sample sets from the same passage and the third set from the previous passage were used.

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Bair

PROVIDER: E-GEOD-45804 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Laccaic acid A is a direct, DNA-competitive inhibitor of DNA methyltransferase 1.

Fagan Rebecca L RL Cryderman Diane E DE Kopelovich Levy L Wallrath Lori L LL Brenner Charles C

The Journal of biological chemistry 20130709 33

Methylation of cytosines in CpG dinucleotides is the predominant epigenetic mark on vertebrate DNA. DNA methylation is associated with transcriptional repression. The pattern of DNA methylation changes during development and with disease. Human DNA methyltransferase 1 (Dnmt1), a 1616-amino acid multidomain enzyme, is essential for maintenance of DNA methylation in proliferating cells and is considered an important cancer drug target. Using a fluorogenic, endonuclease-coupled DNA methylation assa ...[more]

PMID: 23839987

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