Differential Gene Regulation during murine in vivo heart ischemia comparing wildtype and Per2 deficient mice

ABSTRACT: Disruption of peripheral circadian rhyme pathways dominantly leads to metabolic disorders. Studies on circadian rhythm proteins in the heart indicated a role for Clock or Per2 in cardiac metabolism. In fact, Per2-/- mice have larger infarct sizes with a deficient lactate production during myocardial ischemia. To test the hypothesis that cardiac Per2 represents an important regulator of cardiac metabolism during myocardial ischemia, we performed lactate measurements during reperfusion in Per1-/-, Per2-/- or wildtype mice followed by gene array studies using various ischemia-reperfusion protocols comparing wildtype and Per2-/- mice. Lactate measurements in whole blood confirmed a dominant role of Per2 for lactate production during myocardial ischemia. Surprisingly, high-throughput gene array

ORGANISM(S): Mus musculus

SUBMITTER: Tobias Eckle 

PROVIDER: E-GEOD-45818 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress