Characterisation of nuclear architectural alterations during in vitro differentiation of human stem cells of myogenic origin

ABSTRACT: Cell differentiation is based on a synchronised orchestra of complex pathways of intrinsic and extrinsic signals that manifest in the induced expression of specific transcription factors and pivotal genes within the nucleus. One cannot ignore the epigenetic status of differentiating cells, comprising not only histones and DNA modifications but also the spatial and temporal intranuclear chromatin organisation, which is an important regulator of nuclear processes. In the present study, we investigated the nuclear architecture of human primary myoblasts and myocytes in an in vitro culture, with reference to global changes in genomic expression. Repositioning of the chromosomal centromeres, along with alterations in the nuclear shape and volume, was observed as a consequence of myotube formati

ORGANISM(S): Homo sapiens

SUBMITTER: Tomasz Kolanowski 

PROVIDER: E-GEOD-45819 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress