Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription.

ABSTRACT: TFIID is a central player in activated transcription initiation. Recent evidence suggests that the role and composition of TFIID is more diverse than previously understood. To investigate the effects of changing the composition of TFIID in a simple system we depleted TAF1 from Drosophila cells and determined the consequences on metal induced transcription at an inducible gene, Metallothionein B (MtnB). We observe a marked increase in the levels of both the mature message and pre-mRNA in TAF1 depleted cells. Under conditions of continued metal exposure, we show that TAF1 depletion increases the magnitude of the initial transcription burst, but has no effect on the timing of that burst. We also show that TAF1 depletion causes delay in the shut-off of transcription upon removal of the stimulu

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Michael Marr 

PROVIDER: E-GEOD-45873 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress