Nucleosome positioning changes during human embryonic stem cell differentiation.

ABSTRACT: Nucleosomes are the basic unit of chromatin. Nucleosome positioning (NP) plays a key role in transcriptional regulation and other biological processes. To better understand NP we used MNase-seq to investigate changes that occur as human embryonic stem cells (hESCs) transition to nascent mesoderm and then to smooth muscle cells (SMCs). Compared to differentiated cell derivatives, nucleosome occupancy at promoters and other notable genic sites, such as exon/intron junctions and adjacent regions, in hESCs shows a stronger correlation with transcript abundance and is less influenced by sequence content. Upon hESC differentiation, genes being silenced, but not genes being activated, display a substantial change in nucleosome occupancy at their promoters. Genome-wide, we detected a shift of NP t

ORGANISM(S): Homo sapiens

SUBMITTER: wenjuan zhang 

PROVIDER: E-GEOD-45877 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress