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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis of mouse embryonic stem cells treated with MS-275

ABSTRACT: This study describes transcriptome profiling of mouse ES cells treated with MS-275. We included RNA-Seq of ds_cDNA synthesized from double poly(A) selected mRNA RNA-Seq profiling on mouse ES cells treated with MS-275

ORGANISM(S): Mus musculus

SUBMITTER: H.G. Stunnenberg

PROVIDER: E-GEOD-45909 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The class I-specific HDAC inhibitor MS-275 modulates the differentiation potential of mouse embryonic stem cells.

Franci Gianluigi G Casalino Laura L Petraglia Francesca F Miceli Marco M Menafra Roberta R Radic Branka B Tarallo Valeria V Vitale Monica M Scarfò Marzia M Pocsfalvi Gabriella G Baldi Alfonso A Ambrosino Concetta C Zambrano Nicola N Patriarca Eduardo E De Falco Sandro S Minchiotti Gabriella G Stunnenberg Hendrik G HG Altucci Lucia L

Biology open 20130822 10

Exploitation of embryonic stem cells (ESC) for therapeutic use and biomedical applications is severely hampered by the risk of teratocarcinoma formation. Here, we performed a screen of selected epi-modulating compounds and demonstrate that a transient exposure of mouse ESC to MS-275 (Entinostat), a class I histone deacetylase inhibitor (HDAC), modulates differentiation and prevents teratocarcinoma formation. Morphological and molecular data indicate that MS-275-primed ESCs are committed towards ...[more]

PMID: 24167717

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