Expression Profiling in Selenite-induced Proliferation of HuH7 cells

ABSTRACT: Experimental studies confirmed that seleium induced a global change of gene expression in HuH7 cells. Several novally induced secretory growth factors may contribute to the autocrine mode of growth elicited by selenium treatment. Keywords: Treatment HuH7 were cultured in 0.1 μM sodium selenite-containing medium for 15 passages (SELT) or in serum-free medium for 30 h (SF30) or in the regular complete medium containing 10 % fetal calf serum (HUH7) for 30 h. The total RNA were reverse transcried and hybridized to Human 8K-1 (Egenomix Technology Corp., Taiwan). The hybridization time and temperature was 16 h and 62â??, respectively. SELT and HUH7 samples were labeled with Cy5 while SF30 sample was labeled with Cy3. Local background correction and interchip global normalization were accomplis

ORGANISM(S): Homo sapiens

SUBMITTER: Yi-Hsien Chen 

PROVIDER: E-GEOD-4593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress