Global analysis of genes induced during sporulation of Clostridium difficile

ABSTRACT: Purpose: The goal of this study was to identify genes whose expression is induced during sporulation in a Spo0A-, M-OM-^CF-, M-OM-^CE-, M-OM-^CG-, and M-OM-^CK-dependent manner. Methods: Whole genome RNA sequencing was performed on wildtype, spo0A-, sigF-, sigE-, sigG-, and sigK- C. difficile strains (strain 630 background; JIR8094 = parent strain), and the transcriptional profiles of the different mutants during growth on 70:30 agar plates were determined using an Illumina HiSeq1000. Results: This analysis identified 185 genes whose expression is collectively activated by sporulation sigma factors: 150 were M-OM-^CF-dependent, 150 were M-OM-^CE-dependent, 30 were M-OM-^CG-dependent, and 31 were M-OM-^CK-dependent. A total of 237 genes were identified as requiring Spo0A for their express

ORGANISM(S): Clostridium difficile 630

SUBMITTER: Aimee Shen 

PROVIDER: E-GEOD-45977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress