Gene expression analysis of lentivirally-transduced rhesus macaque CD34+ cells long-term following transplant

ABSTRACT: The occurrence of clonal perturbations and leukemia in patients transplanted with retrovirally-transduced autologous hematopoietic stem and progenitor cells (HSPCs) has stimulated extensive investigation, demonstrating that proviral insertions perturb adjacent proto-oncogene expression. Although enhancer-deleted lentiviruses are less likely to result in insertional oncogenesis, there is evidence that they may perturb transcript splicing, and one patient with a benign clonal expansion of lentivirally-transduced HPSC has been reported. The rhesus macaque model provides an opportunity for informative long-term analysis to ask whether transduction impacts on long-term HSPC properties. We utilized two techniques to examine whether lentivirally-transduced HSPCs from eight rhesus macaques transp

ORGANISM(S): Macaca mulatta

SUBMITTER: Yanqin Yang 

PROVIDER: E-GEOD-46032 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress