Synergism between Hedgehog-GLI and EGFR signaling in Hedgehog-responsive human medulloblastoma (Daoy) cells

ABSTRACT: Aberrant activation of Hedgehog (HH) signaling has been identified as a key etiologic factor of many human malignancies. Signal strength, target gene specificity, and oncogenic activity of HH signaling profoundly depend on interactions with other pathways such as epidermal growth factor receptor-mediated signaling which has been shown to cooperate with HH/GLI in basal cell carcinoma and pancreatic cancer. We demonstrate that the human medulloblastoma cell line Daoy possesses a fully inducible endogenous HH pathway. Treatment of Daoy cells with Sonic Hedgehog or Smoothened agonist induced expression of GLI1 protein and prevented processing of GLI3 to its repressor form. To study interactions between HH- and EGF-induced signaling in greater detail, time-resolved measurements were carried o

ORGANISM(S): Homo sapiens

SUBMITTER: Christoph Wierling 

PROVIDER: E-GEOD-46045 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress