Transcriptome analysis of Stem Cells from Human Umbilical Cord Blood

ABSTRACT: Uncovering the molecular mechanism of stem cell self-renewal is critical to broaden current therapeutic applications and to understand how its de-regulation may lead to pathological conditions. In this work, we report changes in gene expression and related cell processes during conditions of high in vitro expansion of CD133+/CD34+ primitive stem cells from human umbilical cord blood (UCB). Based on DNA microarray assays, we demonstrate that primary metabolism and cell cycle genes are specifically up-regulated, as well as members of the Wnt and Notch signaling pathways. Estradiol (E2) treatment resulted in enhanced cell expansion, which was correlated with activation of MEK/ERK signaling genes and inhibition of GLI2, a member of the Hedgehog pathway. Most notably, E2-induced CD133+/CD34+ ce

ORGANISM(S): Homo sapiens

SUBMITTER: Ricardo Vêncio 

PROVIDER: E-GEOD-4609 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress