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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in DN4 thymocytes from mice with an inducible deletion of Ikaros

ABSTRACT: The aim of the experiment was to visualize genes under the control of Ikaros during early T cell development. We used mice carrying floxed Ikaros alleles that were crossed with the Rosa26-CreERT2 mice, and which were injected with tamoxifen for 4 days. DN4 thymocytes were sorted from 4 mice (5-6 week-old) which are positive or not for the Rosa26-CreERT2 transgene. DN4 cells were sorted from 2 Ikf/f Rosa26-CreERT2+ mice and 2 Ikf/f mice, which were all treated with tamoxifen for 4 days. Loss of Ikaros was confirmed in sorted cells from the Ikf/f Rosa26-CreERT2+ mice by western blot. Gene expression profiles were determined on total RNA from sorted cells with Affymetrix Mouse Gene arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Susan Chan

PROVIDER: E-GEOD-46093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression in DN4 thymocytes from mice with an inducible deletion of Ikaros

Project description:The aim of the experiment was to visualize genes under the control of Ikaros during early T cell development. We used mice carrying floxed Ikaros alleles that were crossed with the Rosa26-CreERT2 mice, and which were injected with tamoxifen for 4 days. DN4 thymocytes were sorted from 4 mice (5-6 week-old) which are positive or not for the Rosa26-CreERT2 transgene.

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