Epigenome analysis of DNA modification 5-hydroxymethylcytosine in normal, abnormal, and globozoospermia sperm genomes

ABSTRACT: Genome wide of 5-hydroxymethylcytosine profiling of normal and abnormal, and globozoospermia sperm genomes.Two semen samples were collected from a healthy man and a globozoospermia patient who had consulted a physician at ShengJing Hospital of China Medical University. Other semen samples obtained from two volunteers who were good health generally. 5-hmC enriched genomic DNA libraries were generated following the Illumina protocol for M-bM-^@M-^\Preparing Samples for CHIP sequencing of DNAM-bM-^@M-^]. 100bp single end sequencing on Illumina Hiseq2000 to get 5-hmC-enriched DNA fragment sequence was performed. Examination of 5hmC levels in normal, abnormal, and globozoospermia sperm genomes

ORGANISM(S): Homo sapiens

SUBMITTER: sun baofa 

PROVIDER: E-GEOD-46135 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress